RNase J1

RNase J1, important for the degradation of RNA fragments containing transcription terminators, resolves stalled transcription complexes

BSU14530

BSU_14530

1665

1668

BSU14530

BSU_14530

1,523,118 → 1,524,785

1,523,118 1,524,785

the mutant is strongly impaired in [SW|sporulation], [SW|genetic competence] and many other phenotypes [Pubmed|23504012]

it is not possible to construct a ''[[gene|rnjA]] [[gene|rny]]'' double mutant [Pubmed|23504012]

growth is strongly impaired (doubling time in complex medium 145 minutes vs. 43 minutes for the wild type) [pubmed|26883633]

the mutant is strongly impaired in [SW|sporulation], [SW|genetic competence] and many other phenotypes [Pubmed|23504012]

it is not possible to construct a ''[[gene|rnjA]] [[gene|rny]]'' double mutant [Pubmed|23504012]

endonuclease and 5'-3' exonuclease

degrades [[protein|SR5]] and ''[[gene|gapA]]'' RNAs [pubmed|27449348]

degrades [[gene|yflS ]]mRNA [pubmed|28436820]

endonuclease and 5'-3' exonuclease

degrades [[protein|SR5]] and ''[[gene|gapA]]'' RNAs [pubmed|27449348]

degrades [[gene|yflS ]]mRNA [pubmed|28436820]

resolves stalled transcription complexes by a “torpedo” mechanism, whereby RNase J1 degrades the nascent RNA and causes the transcription complex to disassemble upon collision with [SW|RNA polymerase] [pubmed|31840842]

RNase J subfamily (according to Swiss-Prot)

[SW|metallo-beta-lactamase superfamily] (according to UniProt)

[[protein|RnjB]]

[[this]]

initeraction with [[protein|GapA]]-[[protein|Sr1]] stimulates activity [Pubmed|27449348]

initeraction with [[protein|GapA]]-[[protein|Sr1]] stimulates activity [Pubmed|27449348]

a NAD-cap stabilizes RNA against exonucleolytic attack by RNase J1 [pubmed|30110644]

subject to Clp-dependent proteolysis upon glucose starvation [PubMed|17981983]

required for [[gene|thrS]] RNA processing, involved in maturation of the 5’-end of the 16S rRNA

subject to Clp-dependent proteolysis upon glucose starvation [PubMed|17981983]

required for [[gene|thrS]] RNA processing, involved in maturation of the 5-end of the 16S rRNA

GP41 (''rnjA'' under control of p(xyl)), available in [SW|Jörg Stülke]'s lab

SSB342 (''rnjA'' under pspac), cat, available in [SW|Harald Putzer] lab

GP2506 (Δ[[gene|rnjA]]::''spc''), available in [SW|Jörg Stülke]'s lab

GP41 (''rnjA'' under control of p(xyl)), available in [SW|Jörg Stülke]'s lab

SSB342 (''rnjA'' under pspac), cat, available in [SW|Harald Putzer]'s lab

GP2506 ([[gene|rnjA]]::''spc''), available in [SW|Jörg Stülke]'s lab

for chromosomal expression of RNase J1-Strep (spc): GP1034, available in [SW|Jörg Stülke]'s lab

for chromosomal expression of RNase J1-Strep (cat): GP1042, available in [SW|Jörg Stülke]'s lab

pGP1441: IPTG inducible expression, purification in ''E. coli'' with N-terminal Strep-tag, in [SW|pGP172], available in [SW|Jörg Stülke]'s lab

pGP418 (in [[protein|pAC7]]), available in [SW|Jörg Stülke]'s lab

pGP418 (in [SW|pAC7]), available in [SW|Jörg Stülke]'s lab

[SW|Harald Putzer], IBPC Paris, France [http://www.ibpc.fr/UPR9073/putzer/recherches_harald.htm Homepage]

[SW|David Bechhofer], Mount Sinai School, New York, USA [http://www.mountsinai.org/Research/Centers Laboratories and Programs/Bechhofer Laboratory?citype=Physician&ciid=Bechhofer David H 1255565 Homepage]

[SW|Ciaran Condon], IBPC, Paris, France [http://www.ibpc.fr/UPR9073/condon/index_en.html Homepage]

20458164, 21334965, 21893280, 23403287, 21957024, 22568516, 24064983, 25292357, 27750368

20458164, 21334965, 21893280, 23403287, 21957024, 22568516, 24064983, 25292357, 27750368, 29314657, 29651979, 31464530, 32061882

25940620, 18079181, 19553197, 17981983, 19458035, 15831787, 18204464, 18713320, 19193632, 17005971, 18445592, 17512403, 17229210, 17576666, 19210617, 19633085, 19638340, 19850915, 19880604, 20025672, 20418391, 20572937, 21803996, 21862575, 21925382, 22198292, 22412379, 23504012, 21893285, 21893286, 21908660, 24187087, 26253740, 26802042, 26940229, 27708634, 27449348, 28277929, 28436820, 28542621, 28795788

25940620, 18079181, 19553197, 17981983, 19458035, 15831787, 18204464, 18713320, 19193632, 17005971, 18445592, 17512403, 17229210, 17576666, 19210617, 19633085, 19638340, 19850915, 19880604, 20025672, 20418391, 20572937, 21803996, 21862575, 21925382, 22198292, 22412379, 23504012, 21893285, 21893286, 21908660, 24187087, 26253740, 26802042, 26940229, 27708634, 27449348, 28277929, 28436820, 28542621, 28795788, 26883633, 29242153, 30110644, 30502948, 31840842

for chromosomal expression of RNase J1-Strep (spc): GP1034, available in [SW|Jörg Stülke]'s lab

for chromosomal expression of RNase J1-Strep (cat): GP1042, available in [SW|Jörg Stülke]'s lab

pGP1441: IPTG inducible expression, purification in ''E. coli'' with N-terminal Strep-tag, in [SW|pGP172], available in [SW|Jörg Stülke]'s lab

[SW|Harald Putzer], IBPC Paris, France [http://www.ibpc.fr/UPR9073/putzer/recherches_harald.htm Homepage]

[SW|David Bechhofer], Mount Sinai School, New York, USA [http://www.mountsinai.org/Research/Centers Laboratories and Programs/Bechhofer Laboratory?citype=Physician&ciid=Bechhofer David H 1255565 Homepage]

[SW|Ciaran Condon], IBPC, Paris, France [http://www.ibpc.fr/UPR9073/condon/index_en.html Homepage]